中文 | English
产品分类
·瑞舒伐他汀钙及中间体
·苯磺顺阿曲库铵及中间体
·原料药
·中间体
·Diagnostic & Assay Kits
·标准品
·固化剂
·化合物
产品搜索
联系我们
地址:南京市江宁区天印大道天印慧谷3号商务楼1323
手机:15895913691
电话:+86-25-52719751
传真:+86-25-52719785
客服QQ:2630196394   1839208651
邮箱:admin@chemdiate.com
产品中心  
Alkaline phosphatase Assay Kit

Alkaline phosphatase catalyzes the hydrolysis of phosphate esters under alkaline environments, resulting in the formation of inorganic phosphate and an alcohol. In mammals, this enzyme is found mainly in the liver and bones.  Elevated serum alkaline phosphatase levels are known as hyperalkaline phosphatasemia, whichbeen associated with a variety of symptoms including malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring alkaline phosphatase activity in serum are becoming popular in Research and Drug Discovery.

Chemediate’s Assay Kit is designed to measure alkaline phosphatase activity directly in biological samples without pretreatment. The improved method utilizes phosphorylated fluorescein as the substrate that is hydrolyzed by alkaline phosphatase into a yellow colored and fluorescent product (maximal absorbance around 490 nm, Ex/Em=490/510 nm). The rate of the reaction is directly proportional to the enzyme activity.  The new assay was 50-100 fold more sensitive in an ELISA assay as compared to the conventional assay using the substrate of p-nitrophenol phosphate (PNPP).
Assay Principle

Fluorescent assay of alkaline phosphatase in Elisa assay

Copyright 2012-2021 www.chemdiate.com All rights reserved
南京蓝碧安生化科技有限公司 版权所有 苏ICP备12031533号-1 技术支持:南京做网站
QQ客服