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Esterase assay
 

Lipase is an enzyme secreted by the pancreas and released into the small intestine, where it triggers and aids fat (lipid) breakdown into fatty acids. It converts lipids like glycerides to glycerol and free fatty acids. Pancreatitis occurs due to inflamed pancreas. The onset of pancreatitis is accompanied with an elevation of lipase levels. So monitoring of lipase levels is essential. Pancreatitis is one of the foremost and most widespread pancreas problems. This test is used to diagnose pancreatitis or inflammatory bowel disease. An injured or diseased pancreas will produce abnormal amounts of this enzyme.

Chemediate provides universal fluorogenic assay of esterases and /or lipases via enzymatic hydrolysis of monoacetylated fluorescein methyl ester.  The new assay was advantageous as compared to reported procedures, e.g., compatibility with aqueous solutions without addition of organic co-solvents, and high stability under various biological circumstances.
 

Assay Principle

Figure 1: Fluorescent assay of esterase/lipase using fluorescein derivatives


Figure 2: kinetic monitoring of pig liver esterase catalyzed hydrolysis of fluorescein derivatives in Tris-HCl (pH 7.5)

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