中文 | English
产品分类
·瑞舒伐他汀钙及中间体
·苯磺顺阿曲库铵及中间体
·原料药
·中间体
·Diagnostic & Assay Kits
·标准品
·固化剂
·化合物
产品搜索
联系我们
地址:南京市江宁区天印大道天印慧谷3号商务楼1323
手机:15895913691
电话:+86-25-52719751
传真:+86-25-52719785
客服QQ:2630196394   1839208651
邮箱:admin@chemdiate.com
产品中心  
Peroxidase assay kit
The enzyme horseradish peroxidase (HRP), found in horseradish, is used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule.  For example, an antibody conjugated to HRP may be used to detect a small amount of a specific protein in a western blot. Here, the antibody provides the specificity to locate the protein of interest and the HRP enzyme, in the presence of a substrate, produces a detectable signal. Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry
Upon selected substrates, HRP produces derivatives of the labeled molecule allowing it to be detected and quantified by fluorescence or luminescence.  Chemediate’s Kit uses a non-fluorescent substrate to quantify peroxidase in solution in presence of selected oxidizers Peroxidase catalyzes the oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) in a 1:1 stoichiometry with oxidizer (e.g. hydrogen peroxide) to produce a fluorescent product resorufin (Ex/Em = 560 nm/585 nm or absorbance at 570 nm).  The assay can be performed in a convenient 96-well or 384-well microplate format and is amenable to either fluorescence or absorbance based detection of resorufin.
 
Principle:
Copyright 2012-2021 www.chemdiate.com All rights reserved
南京蓝碧安生化科技有限公司 版权所有 苏ICP备12031533号-1 技术支持:南京做网站
QQ客服